Biological preparations are tested to determine each cell type and the appropriate medium half-life. The complexity of the cell culture medium composition is to optimize a single medium, and the different cell types concerned have highly specific growth requirements. Protein quality. (Povidone) Mammalian cells effectively meet these criteria through their post-translational modification system, which also enables them to become the leading production expression system in the biopharmaceutical industry. The main mammalian expression systems used for the production of biological treatments include NS0 cells, CHO cells and so on. As we all know, cell culture processes and media have important effects on the expression and stability of therapeutic protein products and their key quality attributes (CQA) (including glycosylation, sialylation, charge variation and aggregation, etc.). Optimal cell growth requires monitoring and control of process variables such as pH, dissolved oxygen, dissolved carbon dioxide, and temperature. It can be said that an important and crucial step in cell culture is to select a suitable in vitro growth medium. (Povidone) cell culture medium provides an artificial environment conducive to the survival and proliferation of cultured cells, and maintains the required pH and molecular osmolality. It is usually composed of a complex mixture of amino acids, vitamins, inorganic salts, glucose and serum, and is a source of growth factors for cell growth and protein expression.
It is known that the choice of cell culture media will significantly affect the physical and chemical properties of mAbs. The choice of medium depends on the type of cells to be cultured, as well as the purpose of culture and the difference in the expressed protein, (Povidone). The ingredients present many challenges. Over the years, since the culture medium has had a vital influence on the biological process development of animal cell technology, the formulation of a good medium for culturing mammalian cell reproduction has attracted widespread attention.
The factors that play a role in media design come from three main sources, namely the attributes of the product under consideration, the cell line used for protein production, and the manufacturing process (ie, fed-batch, perfusion or continuous). (Povidone) Due to differences in metabolism, different cell lines have different nutritional requirements, so this factor needs to be properly considered in the process of medium optimization. Although the natural medium consists of only naturally occurring biological fluids and has been proven to be useful and convenient for a variety of animal cell cultures, a key disadvantage is that the lack of precise knowledge of the ingredients may cause the possibility of poor reproducibility . By adding nutrients (such as vitamins, salts, trace elements, carbohydrates and cofactors) of a defined concentration, synthetic or chemical media can be prepared.